Recombinant muis Enolase 1/ENO1-eiwit

Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant mouse Eno1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, c-Myc promoter binding protein

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

Specific activity is > 6,000pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

49.5 kDa (457aa) confirmed by MALDI-TOF

Enolase1, Non-neural enolase, Alpha-Enolase, 0610008I15, AL022784, Eno-1, MBP-1, ATGP3691-10 µg, ATGP3691-20 µg, ATGP3691-50 µg, ATGP3691-100 µg, ATGP3691-250 µg, ATGP3691-500 µg, ATGP3691-1 mg, ATGP3691-10, ATGP3691-20, ATGP3691-50, ATGP3691-100, ATGP3691-250, ATGP3691-500, ATGP3691-1

Plow EF., et al. (2009) Blood. 113 (22) :5371-2. ; ; Bartholomeusz C., et al. (2008) Cancer Res. 68 (22) :9302-10.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.1M NaCl

Metabolism

NP_075608

P17182

Mouse

MSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK