Recombinant muis EOGT-eiwit

EOGT, also known as EGF domain-specific O-linked N-acetylglucosamine transferase, is involved in the regulation of Notch receptor. O-GlcNAc (O-linked b-N-acetylglucosamine) is introduced by a single intracellular O-GlcNAc transferase (OGT) and a single extracellular O-GlcNAc transferase (EOGT) . O-GlcNAc results from the addition of a single N-acetylglucosamine residue to serine/threonine residues. Recombinant mouse EOGT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, A130022J15RiK, Aer61, AI447490, AW214473, AW259391

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

60.4 kDa (516aa)

EOGT, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, A130022J15RiK, Aer61, AI447490, AW214473, AW259391, ATGP3328-10 µg, ATGP3328-20 µg, ATGP3328-50 µg, ATGP3328-100 µg, ATGP3328-250 µg, ATGP3328-500 µg, ATGP3328-1 mg, ATGP3328-10, ATGP3328-20, ATGP3328-50, ATGP3328-100, ATGP3328-250, ATGP3328-500, ATGP3328-1

Hart GW., et al. (2007) Nature 446:1017-1022.; ; Sakaidani Y., et al. (2012) Biochem Biophys Res Commun. 419:14-19.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_780522

Q8BYW9

Mouse

Asp20

DKAHSEADDA PGKALYDYSS LRLPAEHIPF FLHNNRHVAS VCREDSHCPY KKHLENLNYC WGYEKSCAPE FRFGSPVCSY VDLGWTDTLE SAQDMFWRQA DFGYARERLG EIRTICQPER ASDSSLVCSR YLQYCRATGL YLDLRNIKRN HDRFKEDFLQ GGEIGGYCKL DSHALVSEGQ RKSPLQSWFA ELQGYTQLNF RPIEDAKCDI VVEKPTYFMK LDAGINMYHH FCDFLNLYLT QHVNNSFSTD VYIVMWDTST YGYGDLFSDT WKAFTDYDVI HLKTYDSKKV CFKEAVFSLL PRMRYGLFYN TPLISGCQNT GLFRAFSQHV LHRLNITQEG PKDGKVRVTI LARSTEYRKI LNQDELVNAL KTVSTFEVRV VDYKYRELGF LDQLRITHNT DIFIGMHGAG LTHLLFLPDW AAVFELYNCE DERCYLDLAR LRGIHYITWR KPSKVFPQDK GHHPTLGEHP KFTNYSFDVE EFMYLVLQAA EHVLQHPQWP FKKKHDELLE HHHHHH