Recombinant muis Galectine-1/Lgals1-eiwit

LGALS1 is a member of the beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This protein is an autocrine negative growth factor that regulates cell proliferation. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes, thus LGALS1 has immunosuppressive and anti-inflammatory properties. LGALS1 also regulates tumor angiogenesis and is a target for angiostatic cancer therapy. Recombinant mouse LGALS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1, GBP, Galbp, Lect14

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17 kDa (159aa) confirmed by MALDI-TOF

Galectin1, Galectin-1, AA410090; Gal-1; Galbp; galectin-1; L-14.5; L14; Lect14, ATGP1778-10 µg, ATGP1778-20 µg, ATGP1778-50 µg, ATGP1778-100 µg, ATGP1778-250 µg, ATGP1778-500 µg, ATGP1778-1 mg, ATGP1778-10, ATGP1778-20, ATGP1778-50, ATGP1778-100, ATGP1778-250, ATGP1778-500, ATGP1778-1

Ilarregui JM., et al. (2009) . Nat Immunol. 10 (9) :981-91.; ; Gauthier L., Proc Natl Acad Sci u S A. 2002 Oct 1; ;99 (20) :13014-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Cell Cycle

NP_032521

P16045

Mouse

MGSSHHHHHH SSGLVPRGSH MGSHMACGLV ASNLNLKPGE CLKVRGEVAS DAKSFVLNLG KDSNNLCLHF NPRFNAHGDA NTIVCNTKED GTWGTEHREP AFPFQPGSIT EVCITFDQAD LTIKLPDGHE FKFPNRLNME AINYMAADGD FKIKCVAFE