Recombinant muis Galectine-2/Lgals2-eiwit

Galectin 2, also known as Lgals2, belongs to the galectins family. Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. Lgals2 is a monomeric or homodimeric prototype galectin that is expressed in hepatoma, stomach epithelial cells and in colorectal and neural tumors. It induces apoptosis in activated T cells and binds to the cytokine lymphotoxin-a (LTA) with possible implications in risk of myocardial infarction. Human and mouse Lgals2 share approximately 65% amino acid sequence similarity. Recombinant Mouse Lgals2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

The ED50 for this effect is equal or higher than 20 µg/mL. Measured by its ability to agglutinate human red blood cells.

Galectin 2, 2200008F12Rik, AI324147, Gal-2

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

The ED50 for this effect is equal or higher than 20 µg/mL. Measured by its ability to agglutinate human red blood cells.

17.3 kDa (153aa) confirmed by MALDI-TOF

Galectin2, Galectin 2, 2200008F12Rik, AI324147, ATGP3247-10 µg, ATGP3247-20 µg, ATGP3247-50 µg, ATGP3247-100 µg, ATGP3247-250 µg, ATGP3247-500 µg, ATGP3247-1 mg, ATGP3247-10, ATGP3247-20, ATGP3247-50, ATGP3247-100, ATGP3247-250, ATGP3247-500, ATGP3247-1

Mehrabian M., et al. (1993) Genomics. 15:412-420.; ; Cornillot J D., et al. (1998) Glycobiology. 8:425-432.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Signal Transduction

NP_079898

Q9CQW5

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMSEKFEV KDLNMKPGMS LKIKGKIHND VDRFLINLGQ GKETLNLHFN PRFDESTIVC NTSEGGRWGQ EQRENHMCFS PGSEVKITIT FQDKDFKVTL PDGHQLTFPN RLGHNQLHYL SMGGLQISSF KLE