Recombinant muis Galectine-3/LGALS3-eiwit

LGALS3, also known as galectin 3, is a member of the family of animal lectins, which selectively binds beta-galactoside residues. This protein is secreted from cells by ectocytosis, which is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. LGALS3 has been associated with the inhibition of apoptosis and the progression of cancer. It is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. Recombinant mouse LGALS3 protein, used to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

The ED50 for this effect is equal or higher than 25 µg/mL. Measured by its ability to agglutinate human red blood cells.

Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, IgE-binding protein, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Mac-2 antigen, MAC2

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

The ED50 for this effect is equal or higher than 25 µg/mL. Measured by its ability to agglutinate human red blood cells.

29.8 kDa (287aa) confirmed by MALDI-TOF

35 kDa lectin, 35kDa lectin, Carbohydrate binding protein 35, Carbohydrate-binding protein 35, CBP 35, CBP35, Gal 3, Gal3, Gal-3, galactose binding, Galactose specific lectin 3, Galactose-specific lectin 3, GALBP, Galectin 3, Galectin3, GALIG, IgE binding protein, IgE-binding protein, L34 galactoside binding lectin, L-34 galactoside binding lectin, L-34 galactoside-binding lectin, Laminin binding protein, Laminin-binding protein, Lectin, Lectin galactose binding soluble 3, Lectin L29, Lectin L-29, LGALS2, MAC 2, Mac 2 antigen, MAC2, MAC-2, Mac-2 antigen, soluble 3, ATGP3248-10 µg, ATGP3248-20 µg, ATGP3248-50 µg, ATGP3248-100 µg, ATGP3248-250 µg, ATGP3248-500 µg, ATGP3248-1 mg, ATGP3248-010, ATGP3248-020, ATGP3248-050, ATGP3248-100, ATGP3248-250, ATGP3248-500, ATGP3248-01M

Barondes SH., et al. (1994) J Biol Chem. 269 (33) :20807-10.; ; Kadrofske MM., et al. (1998) Arch Biochem Biophys. 349 (1) :7-20.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol,1mM DTT, 2mM EDTA

Cancer

NP_034835.1

Q8C253

Mouse

MADSFSL NDALAGSGNP NPQGYPGAWG NQPGAGGYPG AAYPGAYPGQ APPGAYPGQA PPGAYPGQAP PSAYPGPTAP GAYPGPTAPG AYPGSTAPGA FPGQPGAPGA YPSAPGGYPA AGPYGVPAGP LTVPYDLPLP GGVMPRMLIT IMGTVKPNAN RIVLDFRRGN DVAFHFNPRF NENNRRVIVC NTKQDNNWGK EERQSAFPFE SGKPFKIQVL VEADHFKVAV NDAHLLQYNH RMKNLREISQ LGISGDITLT SANHAMI