Recombinant muis Galectine-4/LGALS4-eiwit

Lgals4, also known as galectin4, belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions, which inhibits chronic inflammations, GVHD, and allergic reactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. Recombinant mouse Lgals4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

The ED50 for this effect is equal or less than5 µg/mL. Measured by its ability to agglutinate human red blood cells.

Galectin-4, Gal-4, Lactose-binding lectin 4

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

The ED50 for this effect is equal or less than 5 µg/mL. Measured by its ability to agglutinate human red blood cells.

38.8 kDa (349aa) confirmed by MALDI-TOF

Gal 4, Gal4, Gal-4, Galectin 4, Galectin4, Galectin-4, LGALS4, LGALS 4, ATGP1784-10 µg, ATGP1784-20 µg, ATGP1784-50 µg, ATGP1784-100 µg, ATGP1784-250 µg, ATGP1784-500 µg, ATGP1784-1 mg, ATGP1784-010, ATGP1784-020, ATGP1784-050, ATGP1784-100, ATGP1784-250, ATGP1784-500, ATGP1784-01M

Huflejt ME., et al. (1997) J Biol Chem. 272 (22) :14294-303. ; ; Huflejt ME., et al. (2004) Glycoconj J. 20 (4) :247-55.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_034836

Q8K419

Mouse

MAYVPAP GYQPTYNPTL PYKRPIPGGL SVGMSVYIQG MAKENMRRFH VNFAVGQDDG ADVAFHFNPR FDGWDKVVFN TMQSGQWGKE EKKKSMPFQK GKHFELVFMV MPEHYKVVVN GNSFYEYGHR LPVQMVTHLQ VDGDLELQSI NFLGGQPAAA PYPGAMTIPA YPAGSPGYNP PQMNTLPVMT GPPVFNPRVP YVGALQGGLT VRRTIIIKGY VLPTARNFVI NFKVGSSGDI ALHLNPRIGD SVVRNSFMNG SWGAEERKVA YNPFGPGQFF DLSIRCGMDR FKVFANGQHL FDFSHRFQAF QMVDTLEING DITLSYVQI