Recombinant muis Galectine-8/LGALS8 eiwit

LGALS8, also known as prostate-specific membrane antigen (PCTA1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates both cell-cell and cell matrix interactions in a manner similar to the selectin subgroup of C-type lectins. Recombinant mouse LGALS8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Measured by its ability to agglutinate human red blood cells. The ED50 range for this effect is ≤ 2 µg/mL.

Lectin galactose binding soluble 8, Lgals-8

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Measured by its ability to agglutinate human red blood cells. The ED50 range for this effect is ≤ 2 µg/mL.

38.6 KDa (339aa) confirmed by MALDI-TOF

1200015E08Rik, AI326142, D13Ertd524e, galactose binding, Lectin, Lectin galactose binding soluble 8, LGALS8, Lgals-8, soluble 8, ATGP1772-10 µg, ATGP1772-20 µg, ATGP1772-50 µg, ATGP1772-100 µg, ATGP1772-250 µg, ATGP1772-500 µg, ATGP1772-1 mg, ATGP1772-010, ATGP1772-020, ATGP1772-050, ATGP1772-100, ATGP1772-250, ATGP1772-500, ATGP1772-01M

Kobayashi T., et al. (2010) Leukemia. 24 (4) :843-50.; ; Streetly MJ., et al. (2010) Blood. 115 (19) :3939-48.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol,1mM DTT

Cancer

NP_061374

Q9JL15

Mouse

MLSLNNL QNIIYNPIIP YVGTITEQLK PGSLIVIRGH VPKDSERFQV DFQLGNSLKP RADVAFHFNP RFKRSSCIVC NTLTQEKWGW EEITYDMPFR KEKSFEIVFM VLKNKFQVAV NGRHVLLYAH RISPEQIDTV GIYGKVNIHS IGFRFSSDLQ SMETSALGLT QINRENIQKP GKLQLSLPFE ARLNASMGPG RTVVIKGEVN TNARSFNVDL VAGKTRDIAL HLNPRLNVKA FVRNSFLQDA WGEEERNITC FPFSSGMYFE MIIYCDVREF KVAINGVHSL EYKHRFKDLS SIDTLSVDGD IRLLDVRSW