Recombinant muis Glutardoxin 1/GLRX1-eiwit

Glrx, also known as glutaredoxin-1, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Mammalian Glutaredoxin is known to have two isoforms, GRX1 and GRX2. GRX1 is a cytosolic protein, whereas GRX2 is localized both in the mitochondria and nucleus. Glrx may be involved in a various cellular events such as signal transduction, stress response, and metabolic regulation by regulating the redox status of cellular proteins. Recombinant mouse Glrx protein was expressed in E. coli and purified by using conventional chromatography techniques.

Glutaredoxin-1, C86710, D13Wsu156e, Glrx1, Grx1, Ttase, Thioltransferase-1

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

14.3 kDa (130aa) confirmed by MALDI-TOF

Glutaredoxin1, GLRX1, Glutaredoxin-1, C86710, D13Wsu156e, Glrx1, Grx1, Ttase, ATGP3698-10 µg, ATGP3698-20 µg, ATGP3698-50 µg, ATGP3698-100 µg, ATGP3698-250 µg, ATGP3698-500 µg, ATGP3698-1 mg, ATGP3698-10, ATGP3698-20, ATGP3698-50, ATGP3698-100, ATGP3698-250, ATGP3698-500, ATGP3698-1

Reynaert NL., et al. (2006) Proc Natl Acad Sci U S A. 103 (35) :13086-91.; ; Kanda M., et al. (2006) J Biol Chem. 281 (39) :28518-28.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 5.0) containing 20% glycerol, 0.1M NaCl

Redox Proteins

NP_444338

Q9QUH0

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMAQEFVN CKIQSGKVVV FIKPTCPYCR KTQEILSQLP FKQGLLEFVD ITATNNTSAI QDYLQQLTGA RTVPRVFIGK DCIGGCSDLI SMQQTGELMT RLKQIGALQL