Recombinant muis Glutathion S-transferase A1/GSTA1-eiwit

Gsta1 also known as Glutathione S-transferase, A1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant mouse Gsta1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Glutathione S-transferase, A1, Gst2-1, OTTMUSG00000031890

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 4,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

28 kDa (246aa) confirmed by MALDI-TOF

Gsta1, Glutathione S-transferase A1, Glutathione S-transferase, A1, Gst2-1, OTTMUSG00000031890, ATGP3086-10 µg, ATGP3086-20 µg, ATGP3086-50 µg, ATGP3086-100 µg, ATGP3086-250 µg, ATGP3086-500 µg, ATGP3086-1 mg, ATGP3086-10, ATGP3086-20, ATGP3086-50, ATGP3086-100, ATGP3086-250, ATGP3086-500, ATGP3086-1

Grahn, E., et al. (2006) Acta Cryst. 62 (2) : 197-207.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Metabolism

NP_032207

P13745

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMAGKPVL HYFNARGRME CIRWLLAAAG VEFEEKFIQS PEDLEKLKKD GNLMFDQVPM VEIDGMKLAQ TRAILNYIAT KYDLYGKDMK ERALIDMYSE GILDLTEMIG QLVLCPPDQR EAKTALAKDR TKNRYLPAFE KVLKSHGQDY LVGNRLTRVD IHLLEVLLYV EEFDASLLTP FPLLKAFKSR ISSLPNVKKF LQPGSQRKPP MDAKQIQEAR KAFKIQ