Recombinant muis Glutathion S-transferase mu 1/GSTM1-eiwit

GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur) . The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 40,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

PmGT10, GSTM1, GST1, GST mu, GST class mu 1, GST class Mu, GST 1-1, Glutathione S-transferase Mu 1, Glutathione S transferase mu

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 40,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

28.1 kDa (238aa) confirmed by MALDI-TOF

PmGT10, GSTM-1, GSTM1, GSTM 1, GST-1, GST1, GST mu, GST class-mu1, GST class-mu 1, GST class mu1, GST class mu 1, GST class mu, GST 1-1, GST 1 1, GST 1, Glutathione S-Transferase Mu1, Glutathione S-transferase Mu 1, Glutathione S Transferase Mu 1, Glutathione S transferase mu, ATGP0413-10 µg, ATGP0413-20 µg, ATGP0413-50 µg, ATGP0413-100 µg, ATGP0413-250 µg, ATGP0413-500 µg, ATGP0413-1 mg, ATGP0413-010, ATGP0413-020, ATGP0413-050, ATGP0413-100, ATGP0413-250, ATGP0413-500, ATGP0413-01M

Goekkurt E., et al. (2009) J Clin Oncol. 27 (17) :2863-73. ; ; Koutros S., et al. (2009) Cancer Res. 69 (5) :1877-84.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Enzymes & Proteases

NP_034488

P10649

Mouse

MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK