Recombinant muis Glutathion S-Transferase Pi 1/GSTP1-eiwit

Gstp1 also known as Glutathione S-transferase P 1. Gstp1 is a superfamily of ubiquitously expressed cytosolic, mitochondrial and membrane-associated enzymes involved in a range of detoxification processes. Also Increased Gstp1 levels were consistently associated with reduced Cdk5 activity. Gstp1 directly inhibits Cdk5 by dislodging p25/p35, and indirectly by eliminating oxidative stress. Recombinant mouse Gstp1, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 40 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Glutathione S-transferase P 1

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

Specific activity is > 40unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

26 kDa (233aa) confirmed by MALDI-TOF

Gstp1, Glutathione S-transferase P 1, ATGP3146-10 µg, ATGP3146-20 µg, ATGP3146-50 µg, ATGP3146-100 µg, ATGP3146-250 µg, ATGP3146-500 µg, ATGP3146-1 mg, ATGP3146-10, ATGP3146-20, ATGP3146-50, ATGP3146-100, ATGP3146-250, ATGP3146-500, ATGP3146-1

Sailer V., et al. (2015) Histopathology. doi: 10.1111/his.12671.; ; S Kai-Hui., et al. (2011) Journal of Neurochemistry vol. 118 (5) : 902-914.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_038569

P19157

Mouse

MPPYTIV YFPVRGRCEA MRMLLADQGQ SWKEEVVTID TWMQGLLKPT CLYGQLPKFE DGDLTLYQSN AILRHLGRSL GLYGKNQREA AQMDMVNDGV EDLRGKYVTL IYTNYENGKN DYVKALPGHL KPFETLLSQN QGGKAFIVGD QISFADYNLL DLLLIHQVLA PGCLDNFPLL SAYVARLSAR PKIKAFLSSP EHVNRPINGN GKQ