Recombinant muis Glyoxalase I-eiwit

Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. Recombinant mouse Glo1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 micromol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.

Glyoxalase 1, Lactoylglutathione lyase, 0610009E22Rik, 1110008E19Rik, 2510049H23Rik, AW550643, Glo-1, Glo-1r, Glo-1s, Glo1-r, Glo1-s, GLY1, Qglo

Baculovirus

Mouse

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 210unit/mg, and is defined as the amount of enzyme that will form 1.0 micromol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.

21.8 kDa (192aa)

Glyoxalase I, Glyoxalase 1, Lactoylglutathione lyase, 0610009E22Rik, 1110008E19Rik, 2510049H23Rik, AW550643, Glo-1, Glo-1r, Glo-1s, Glo1-r, Glo1-s, GLY1, Qglo, ATGP3342-10 µg, ATGP3342-20 µg, ATGP3342-50 µg, ATGP3342-100 µg, ATGP3342-250 µg, ATGP3342-500 µg, ATGP3342-1 mg, ATGP3342-10, ATGP3342-20, ATGP3342-50, ATGP3342-100, ATGP3342-250, ATGP3342-500, ATGP3342-1

Kawatani M., et al. (2008) Proc Natl Acad Sci U S A. 105:11691-11696.; ; Kromer SA., et al. (2005) J Neurosci. 25:4375-4384.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_079650

Q9CPU0

Mouse

Met1

MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH