Recombinant muis GOT2-eiwit

GOT2, also known aspartate aminotransferase, mitochondrial, belongs to the class-IPyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant mouse GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specific activity is > 20 units/mg, and is defined as the amount of enzyme that converts 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

AATM_HUMAN, AL022787, Aspartate aminotransferase, Aspartate aminotransferase 2, Aspartate aminotransferase, mitochondrial, Aspartate transaminase 2, ASPATA, EC 2.6.1.1, FABP 1, FABP pm, FABP-1, FABPpm, Fatty acid binding protein, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, Glutamate oxaloacetate transaminase 2, mitochondrial, Glutamate oxaloacetate transaminase, mitochondrial, Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2), Got 2, GOT2, KAT4, KATIV, kynurenine aminotransferase 4, Kynurenine aminotransferase IV, kynurenine-oxoglutarate transaminase 4, kynurenine-oxoglutarate transaminase IV, mAspAT, MGC102129, MGC115763, mitAAT, mitochondrial, Mitochondrial aspartate aminotransferase, OTTMUSP00000017748, Plasma membrane fatty acid binding protein, Plasma membrane-associated fatty acid-binding protein, Transaminase A

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 20unit/mg, and is defined as the amount of enzyme that converts 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

46.8 kDa (422aa) confirmed by MALDI-TOF

GOT2, ATGP3882-10 µg, ATGP3882-20 µg, ATGP3882-50 µg, ATGP3882-100 µg, ATGP3882-250 µg, ATGP3882-500 µg, ATGP3882-1 mg, ATGP3882-10, ATGP3882-20, ATGP3882-50, ATGP3882-100, ATGP3882-250, ATGP3882-500, ATGP3882-1

Honorat JA., et al. (2017) PLoS One. 12 (11) :e0187215.; ; Han Q., et al. (2010) BMC Biochemistry. 11:19.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_034455

P05202

Mouse

SSWWTHVEM GPPDPILGVT EAFKRDTNSK KMNLGVGAYR DDNGKPYVLP SVRKAEAQIA AKNLDKEYLP IGGLAEFCKA SAELALGENN EVLKSGRFVT VQTISGTGAL RVGASFLQRF FKFSRDVFLP KPSWGNHTPI FRDAGMQLQG YRYYDPKTCG FDFSGALEDI SKIPEQSVLL LHACAHNPTG VDPRPEQWKE IASVVKKKNL FAFFDMAYQG FASGDGDKDA WAVRHFIEQG INVCLCQSYA KNMGLYGERV GAFTVVCKDA EEAKRVESQL KILIRPLYSN PPLNGARIAA TILTSPDLRK QWLQEVKGMA DRIISMRTQL VSNLKKEGSS HNWQHITDQI GMFCFTGLKP EQVERLTKEF SVYMTKDGRI SVAGVTSGNV GYLAHAIHQV TK