Recombinant muis Granzyme B-eiwit

Granzyme B, also known as Gzmb, is a member of the granzyme family of cell death-inducing serine proteases specially expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. It plays crucial role for target cell lysis in cell-mediated immune responses. This protein is linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. It cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Recombinant mouse Granzyme B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Gzmb, CTLA-1, Cytotoxic cell protease 1, CCP1, Fragmentin-2, Ctla-1, Ctla1, AI553453, CCP-1/C11, CCP1, GZB

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 9,000pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

26.3 kDa (235aa)

Granzyme B, ATGP3913-10 µg, ATGP3913-20 µg, ATGP3913-50 µg, ATGP3913-100 µg, ATGP3913-250 µg, ATGP3913-500 µg, ATGP3913-1 mg, ATGP3913-10, ATGP3913-20, ATGP3913-50, ATGP3913-100, ATGP3913-250, ATGP3913-500, ATGP3913-1

D' Eliseo D., et al. (2016) J Exp Clin Cancer Res. 35:24.; ; Rabani M., et al. (2018) Clin Immunol. 188:45-51.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Apoptosis

NP_038570

P04187

Mouse

Gly19