Recombinant muis Hemoglobine zeta/HBZ eiwit

Hba-x, also known as Hemoglobin subunit zeta (Hbz), is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The Hba-x polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. Hba-x gene includes five functional genes and two pseudogenes. The order of genes is: 5' -zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant mouse Hba-x fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hemoglobin subunit zeta, Alpha-like embryonic globin chain x, Hemoglobin zeta chain, Zeta-globin, HBZ1, HBZ-T1, Hba-x

E.coli

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

18.3 kDa (162aa) confirmed by MALDI-TOF

Hba-x, Hemoglobin subunit zeta, AI450015, Hbz, ATGP3740-10 µg, ATGP3740-20 µg, ATGP3740-50 µg, ATGP3740-100 µg, ATGP3740-250 µg, ATGP3740-500 µg, ATGP3740-1 mg, ATGP3740-10, ATGP3740-20, ATGP3740-50, ATGP3740-100, ATGP3740-250, ATGP3740-500, ATGP3740-1

Lau ET., et al. (2001) Prenat. Diagn. 21 (7) : 529-39; ; Kidd RD., et al. (2001) Biochemistry. 40 (51) :15669-75

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 6.0) containing 0.1M NaCl, 10% glycerol

Immunology

NP_034535

P06467

Mouse

MGSSHHHHHH SSGLVPRGSH MSLMKNERAI IMSMWEKMAA QAEPIGTETL ERLFCSYPQT KTYFPHFDLH HGSQQLRAHG FKIMTAVGDA VKSIDNLSSA LTKLSELHAY ILRVDPVNFK LLSHCLLVTM AARFPADFTP EVHEAWDKFM SILSSILTEK YR