Recombinant muis HPD-eiwit

Hpd, also known as 4-hydroxyphenylpyruvate dioxygenase, is a Fe-containing enzyme that catalyzes the second reaction in the catabolism of tyrosine the conversion of 4-hydroxyphenylpyruvate to homogentisate. Existing as a homodimer, Hpd uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. Defects in the gene encoding Hpd are the cause of tyrosinemia type 3 and hawkinsinuria, both of which are inborn errors of metabolism that are associated with a variety of symptoms, including mental retardation and seizures and hair and urine abnormalities. Recombinant mouse Hpd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

4-hydroxyphenylpyruvate dioxygenase, 4HPPD, Fla, Flp, Hppd, Laf, 4-hydroxyphenylpyruvic acid oxidase, HPPDase, F Alloantigen, F protein

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

47.4 kDa (416aa) confirmed by MALDI-TOF

Hpd, 4-hydroxyphenylpyruvate dioxygenase, 4HPPD, Fla, Flp, Hppd, Laf, ATGP3760-10 µg, ATGP3760-20 µg, ATGP3760-50 µg, ATGP3760-100 µg, ATGP3760-250 µg, ATGP3760-500 µg, ATGP3760-1 mg, ATGP3760-10, ATGP3760-20, ATGP3760-50, ATGP3760-100, ATGP3760-250, ATGP3760-500, ATGP3760-1

Wada GH., et al. (1975) J Biol Chem J. 250 (17) :6720-6.; ; Endo F., et al. (1997) J Biol Chem. 26; ;272 (39) :24426-32.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_032303

P49429

Mouse

MTTYNNK GPKPERGRFL HFHSVTFWVG NAKQAASFYC NKMGFEPLAY RGLETGSREV VSHVIKQGKI VFVLCSALNP WNKEMGDHLV KHGDGVKDIA FEVEDCDHIV QKARERGAKI VREPWVEQDK FGKVKFAVLQ TYGDTTHTLV EKINYTGRFL PGFEAPTYKD TLLPKLPRCN LEIIDHIVGN QPDQEMQSAS EWYLKNLQFH RFWSVDDTQV HTEYSSLRSI VVTNYEESIK MPINEPAPGR KKSQIQEYVD YNGGAGVQHI ALKTEDIITA IRHLRERGTE FLAAPSSYYK LLRENLKSAK IQVKESMDVL EELHILVDYD EKGYLLQIFT KPMQDRPTLF LEVIQRHNHQ GFGAGNFNSL FKAFEEEQAL RGNLTDLEPN GVRSGM