Recombinant muis Hydroxyzuur Oxidase-1/HAO-1 eiwit

Hydroxyacid Oxidase-1/HAO-1, also known as hydroxyacid oxidase 1, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on two carbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specific activity is > 1,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C

(S) -2-hydroxy-acid oxidase; EC 1.1.3.15, Glycolate oxidase, GOX, GOX1 mgC142227; GOXMGC142225, HAO1, HAO-1, HAOX1, hydroxyacid oxidase (glycolate oxidase) 1, hydroxyacid oxidase 1, Hydroxyacid Oxidase1, Hydroxyacid Oxidase-1

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 1,000pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C

43.4 kDa (393aa) confirmed by MALDI-TOF

Hydroxyacid Oxidase1, HAO1, ATGP3870-10 µg, ATGP3870-20 µg, ATGP3870-50 µg, ATGP3870-100 µg, ATGP3870-250 µg, ATGP3870-500 µg, ATGP3870-1 mg, ATGP3870-10, ATGP3870-20, ATGP3870-50, ATGP3870-100, ATGP3870-250, ATGP3870-500, ATGP3870-1

Pennati A., et al. (2009) J. Biol. Chem. 284 (45) :31214-22.; ; Murray MS., et al. (2008) Biochemistry. 47 (8) :2439-49

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_034533

Q9WU19

Mouse

MLPRLVC ISDYEQHVRS VLQKSVYDYY RSGANDQETL ADNIQAFSRW KLYPRMLRNV ADIDLSTSVL GQRVSMPICV GATAMQCMAH VDGELATVRA CQTMGTGMML SSWATSSIEE VAEAGPEALR WMQLYIYKDR EISRQIVKRA EKQGYKAIFV TVDTPYLGNR IDDVRNRFKL PPQLRMKNFE TNDLAFSPKG NFGDNSGLAE YVAQAIDPSL SWDDITWLRR LTSLPIVVKG ILRGDDAKEA VKHGVDGILV SNHGARQLDG VPATIDVLPE IVEAVEGKVE VFLDGGVRKG TDVLKALALG AKAVFVGRPI IWGLAFQGEK GVQDVLEILK EEFRLAMALS GCQNVKVIDK TLVRKNPLAV SKI