Recombinant muis IgG2A-eiwit

Immunoglobulin G (IgG) is antibody molecules. Each IgG is composed of four peptide chains -two heavy chains gamma and two light chains. Each IgG has two antigen binding sites. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. It also plays an important role in Antibody-dependent cell-mediated cytotoxicity (ADCC) and Intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. Recombinant mouse IgG protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Immunoglobulin heavy chain constant region gamma 2a, Immunoglobulin G

Baculovirus

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.5 kDa (242aa)

IgG2A, Immunoglobulin heavy chain constant region gamma 2a, Immunoglobulin G, ATGP1512-10 µg, ATGP1512-20 µg, ATGP1512-50 µg, ATGP1512-100 µg, ATGP1512-250 µg, ATGP1512-500 µg, ATGP1512-1 mg, ATGP1512-10, ATGP1512-20, ATGP1512-50, ATGP1512-100, ATGP1512-250, ATGP1512-500, ATGP1512-1

Mallery DL, et al. (2010) Proc. Natl. Acad. Sci. u.S.A. 107 (46) : 19985-19990.; ; Stadlmann J, et al. (2008) Proteomics 8 (14) : 2858-2871.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Immunology

CAC20702

P01863

Mouse

EPRGPTI KPCPPCKCPA PNLLGGPSVF IFPPKIKDVL MISLSPIVTC VVVDVSEDDP DVQISWFVNN VEVHTAQTQT HREDYNSTLR VVSALPIQHQ DWMSGKEFKC KVNNKDLPAP IERTISKPKG SVRAPQVYVL PPPEEEMTKK QVTLTCMVTD FMPEDIYVEW TNNGKTELNY KNTEPVLDSD GSYFMYSKLR VEKKNWVERN SYSCSVVHEG LHNHHTTKSF SRTPGK