Recombinant muis IL-1 alfa/IL1A eiwit

IL-1 alpha /IL-1F1, also known as interleukin-1 alpha, is a member of the interleukin-1 cytokine family. Cytokines are protein signaling compounds that are the main mediators of the immune response. This protein is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. Also, it stimulates thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation. Recombinant mouse IL-1 alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Mouse IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.15 ng/mL.

Interleukin-1 alpha precursor, Interleukin 1 alpha, IL-1F1, IL-1A, IL-1 alpha

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.15 ng/mL.

19.0kDa (165aa)

ATGP3778, Pro-interleukin-1-alpha, Pro interleukin 1 alpha, Preinterleukin 1 alpha, Interleukin-1 alpha precursor, Interleukin 1 alpha, IL1F1, IL-1a, IL1a, IL-1 alpha, IL1 alpha, IL-1 a, IL1 a, IL 1 alpha, IL 1 a, Hematopoietin-1, Hematopoietin1, Hematopoietin 1, ATGP3932-10 µg, ATGP3932-20 µg, ATGP3932-50 µg, ATGP3932-100 µg, ATGP3932-250 µg, ATGP3932-500 µg, ATGP3932-1 mg, ATGP3932-010, ATGP3932-020, ATGP3932-050, ATGP3932-100, ATGP3932-250, ATGP3932-500, ATGP3932-01M, ATGP3778

Dinarello CA., et al. (1997) Semin Oncol. 24:S9-81-S9-93.; ; Bankers-Fulbright JL., et al. (1996) Life Sci. 59:61-83.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_034684

P01582

Mouse

Ala

SAPYTYQ SDLRYKLMKL VRQKFVMNDS LNQTIYQDVD KHYLSTTWLN DLQQEVKFDM YAYSSGGDDS KYPVTLKISD SQLFVSAQGE DQPVLLKELP ETPKLITGSE TDLIFFWKSI NSKNYFTSAA YPELFIATKE QSRVHLARGL PSMTDFQIS