Recombinant muis IL-15 eiwit

Interleukin (IL) -15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma (c) -expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxicT-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Recombinant Mouse IL-15 was expressed in E. coli and purified by using conventional chromatography techniques.

Mouse IL-15 stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range is less or equal to 20 ng/mL.

Interleukin-15

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 20 ng/mL.

17.6 kDa (152aa) confirmed by MALDI-TOF

IL15, IL 15, IL-15, Interleukin 15, Interleukin15, Interleukin-15, AI503618, MGC9721, IL-T, Interleukin-15 precursor, OTTHUMP00000164617, ATGP3883-10 µg, ATGP3883-20 µg, ATGP3883-50 µg, ATGP3883-100 µg, ATGP3883-250 µg, ATGP3883-500 µg, ATGP3883-1 mg, ATGP3883-010, ATGP3883-020, ATGP3883-050, ATGP3883-100, ATGP3883-250, ATGP3883-500, ATGP3883-01M

J G Giri., et al. (1994) EMBO. 13 (12) : 2822-2830.; ; Lorenzen I., et al. (2006) . J Biol Chem. 281 (10) :6642-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_032383

P48346

Mouse

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