Recombinant muis IL-1Ra/IL1RN-eiwit

Interleukin 1 receptor antagonist (IL1RN), also known as IL1-RA, is a member of the interleukin 1 cytokine family. IL1RN may represent a reservoir of IL-1 inhibitors, released upon cell death, whose function is no limit the pro-inflammatory signals but most prominently by mononuclear phagocytes and keratinocytes. IL1RN is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Recombinant mouse IL1RN protein, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Mouse IL-1ra/IL-1F3 inhibits Mouse IL-1 alpha induced cell proliferation in the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 60 ng/mL.

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RA

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/mL with Mouse IL-1 alpha.

20 kDa (177aa) confirmed by MALDI-TOF

ATGP1516, IL1ra, ICIL-1RA, IL1F3, ATGP3884-10 µg, ATGP3884-20 µg, ATGP3884-50 µg, ATGP3884-100 µg, ATGP3884-250 µg, ATGP3884-500 µg, ATGP3884-1 mg, ATGP3884-010, ATGP3884-020, ATGP3884-050, ATGP3884-100, ATGP3884-250, ATGP3884-500, ATGP3884-01M, ATGP1516

Steinkasserer A., et al. (1992) Genomics. 13 (3) :654-7.; ; Langdahl BL., et al. (2000) J Bone Miner Res. 15 (3) : 403-14.

Hiramoto K, et al. The role of the active oxygen produced from Gp91phox NADPH oxidase on the newborn weight of mouse pups. (Biology and Medicine. 2015) {https://go.gale.com/ps/i.do?id=GALE%7CA440917547&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=09748369&p=HRCA&sw=w&userGroupName=anon%7E4b2d7215&aty=open-web-entry}; ; Hiramoto K, et al. Gp91phox NADPH oxidase modulates litter size by regulating mucin1 in the uterus of mice. (Syst Biol Reprod Med. 2017) {https://pubmed.ncbi.nlm.nih.gov/28301257/}; ; Hiramoto K, et al. The Role of gp91phox and the Effect of Tranexamic Acid Administration on Hair Color in Mice. (Int J Mol Sci. 2019) {https://pubmed.ncbi.nlm.nih.gov/31151207/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Hormones & Cytokines

NP_001034790

P25085

Mouse

RPSGK RPCKMQAFRI WDTNQKTFYL RNNQLIAGYL QGPNIKLEEK IDMVPIDLHS VFLGIHGGKL CLSCAKSGDD IKLQLEEVNI TDLSKNKEED KRFTFIRSEK GPTTSFESAA CPGWFLCTTL EADRPVSLTN TPEEPLIVTK FYFQEDQ