Recombinant muis IL-3 eiwit

IL3, also known as interleukin 3, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, it also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Recombinant mouse IL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured in a cell proliferation assay using BaF3 mouse pro-B cells. The ED50 range ≤0.1 ng/mL.

IL-3, Multipotential colony-stimulating factor (multi-CSF), Mast cell growth factor (MCGF), Interleukin 3 (colony-stimulating factor, multiple), MuLTI-CSF, MCGF, Multilineage-colony-stimulating factor, Hematopoietic growth factor, P-cell stimulating factor, Mast-cell growth factor

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using BaF3 mouse pro-B cells. The ED50 range ≤0.1 ng/mL.

16.7 kDa (148aa)

IL3, ATGP3211-10 µg, ATGP3211-20 µg, ATGP3211-50 µg, ATGP3211-100 µg, ATGP3211-250 µg, ATGP3211-500 µg, ATGP3211-1 mg, ATGP3211-10, ATGP3211-20, ATGP3211-50, ATGP3211-100, ATGP3211-250, ATGP3211-500, ATGP3211-1

Yokota. T., et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070.; ; Miyatake., S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316.; ; Knepper T.P., et al. (1992) Biochemistry 31:11651-11659.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_034686

P01586

Mouse