Recombinant muis IL-6 eiwit

Il6, also known as Interleukin-6, is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. This protein plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. Recombinant mouse Il6, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED50 for this effects is less or equal to 0.1 ng/mL.

Interleukin 6, IL-6, B-cell hybridoma growth factor, BSF, Interleukin HP-1

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED50 for this effects is less or equal to 0.1 ng/mL.

22.5 kDa (193aa) confirmed by MALDI-TOF

Interleukin-6, Interleukin 6, IL-6, il6, il 6, IFN-beta-2, Ifnb2, CDF, ATGP3154-10 µg, ATGP3154-20 µg, ATGP3154-50 µg, ATGP3154-100 µg, ATGP3154-250 µg, ATGP3154-500 µg, ATGP3154-1 mg, ATGP3154-010, ATGP3154-020, ATGP3154-050, ATGP3154-100, ATGP3154-250, ATGP3154-500, ATGP3154-01M

May LT. et al., (1991) Cytokine 3:204-211.; ; Fiorillo MT. et al., (1992) Eur. J. Immunol. 22:799-804.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_112445

P08505

Mouse