Recombinant muis IMPAD1-eiwit

IMPAD1, also known as inositol monophosphatase 3, may play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3, 5-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Recombinant mouse IMPAD1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 5,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze Adenosine 3, 5-diphosphate per minute at pH 7.5 at 25C.

Inositol monophosphatase 3, IMPAD1, 1110001C20Rik, AA408880, AI451589, AL022796, B230207P20, gPAPP, Jaws

Baculovirus

Mouse

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 5,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze Adenosine 3, 5-diphosphate per minute at pH 7.5 at 25C.

36.2 kDa (332aa)

IMPAD1, Inositol monophosphatase 3, IMPAD1, 1110001C20Rik, AA408880, AI451589, AL022796, B230207P20, gPAPP, Jaws, ATGP3497-10 µg, ATGP3497-20 µg, ATGP3497-50 µg, ATGP3497-100 µg, ATGP3497-250 µg, ATGP3497-500 µg, ATGP3497-1 mg, ATGP3497-10, ATGP3497-20, ATGP3497-50, ATGP3497-100, ATGP3497-250, ATGP3497-500, ATGP3497-1

Strott CA. (2002) Endocr Rev. 23:703-732.; ; Frederick JP., et al. (2008) Proc Natl Acad Sci U S A. 105:11605-11612.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_808398

Q80V26

Mouse

Ala

ADPGRFSLFG LGSEPAAGEA EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP VLGVIHKPFS EYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH