Recombinant muis Kell eiwit

Kell, also known as Kell blood group glycoprotein homolog, is a member of the zinc endopeptidase of the neprilysin (NEP) family. It is an enzyme whose principal known function is the production of a potent bioactive peptide, ET-3. It also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins. It is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens. The Kell antigen system (also known as Kell–Cellano system) is one of the most clinically important blood group systems. Recombinant mouse Kell, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Kell blood group antigen, kell blood group glycoprotein, Kell blood group, Kell blood group glycoprotein homolog, KEL, Kell, CD238 antigen, CD238, ECE3, Kell blood group-metalloendopeptidase, Kell blood group-metallo-endopeptidase

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

76.3kDa (674aa)

, ATGP3955-10 µg, ATGP3955-20 µg, ATGP3955-50 µg, ATGP3955-100 µg, ATGP3955-250 µg, ATGP3955-500 µg, ATGP3955-1 mg, ATGP3955-10, ATGP3955-20, ATGP3955-50, ATGP3955-100, ATGP3955-250, ATGP3955-500, ATGP3955-1

Lee, S. et al. (1999) Blood 94:1440-1450.; ; Lee, S. et al. (2000) Transfus. Med. Rev. 14:93-103.; ; Turner, A.J. et al. (2001) BioEssays. 23:261-269.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_115929

Q9EQF2

Mouse

Ala49

IFRNCGP CPCETPVCME LLDHYLASGN RSVAPCTDFF SFACEKANGT SDSFQALTEE NKSRLWRLLE APGSWHLGSG EEKAFQFYNS CMDTDAIEAS GSGPLIQIIE ELGGWNITGN WTSLDFNQNL RLLMSQYGHF PFFRAYLRPH PAPPHTPIIQ IDQPEFDILL QQEQEQKVYA QILREYVTYL NRLGTLLGSN PQEAQQHASW SIVFTSRLFQ FLRPQQQQQA QDKLFHVVTI DELQEMAPAI DWLSCLQAIF TPMSLNSSQT LVVHDLDYLR NMSQLVEEGL LNHRESIQSY MILGLVDTLS PALDTKFQEA RRELIQELRK LKERPPLPAY PRWMKCVEQT GAFFEPTLAA LFVREAFGPS IQSAAMELFA EIKDAVIIRL KKLSWISEET QKEALNKLAQ LQVEMGAPKR AVKPDIATQE YNDIQLGPSF LQSFLSCVRS LRARNVQSFL QPFPYHRWQK SPWEVNAYYS ISDHMVVFPA GLLQPPFFHP GYPRAVNFGA AGSIMAHELL HIFYQLLLPG GCPACDTHVL QEALLCLERH YAAFPLPSIS SFNGSHTLLE NAADIGGVAI AFQAYSKRIV EHTGELTLPN LDLSPYQLFF RSYAQVMCRG LSSQDPQDPH SPPSLRVHGP LSNTPDFAKH FHCPRGTLLN PSARCKLW