Recombinant muis Lactaatdehydrogenase B/LDHB-eiwit

Ldhb, also known as L-lactate dehydrogenase B chain isoform Ldhb, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD) . The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant mouse Ldhb protein, fused to His-tagging at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 150 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2, LDH heart subunit

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 150unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

39 kDa (357aa) confirmed by MALDI-TOF

L-lactate dehydrogenase B chain, Ldhb, L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2, ATGP3643-10 µg, ATGP3643-20 µg, ATGP3643-50 µg, ATGP3643-100 µg, ATGP3643-250 µg, ATGP3643-500 µg, ATGP3643-1 mg, ATGP3643-10, ATGP3643-20, ATGP3643-50, ATGP3643-100, ATGP3643-250, ATGP3643-500, ATGP3643-1

Read JA., et al. (2001) Proteins. 43 (2) :175-85.; ; Butt AA., et al. (2002) AIDS Read. 12 (7) :317-21

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Cancer

NP_032518

P16125

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL