Recombinant muis Legumaisparaginyl Endopeptidase-eiwit

LGMN, also known as legumain, is a lysosomal cysteine protease found in all mouse tissues, but was particularly abundant in kidney and placenta. It plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases. Over expression of Legumain in tumors is significant for invasion/metastasis. Recombinant mouse LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Legumain, AEP, AI746452, AU022324, Prsc1

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

48.6 kDa (426aa)

Legumain, Asparaginyl Endopeptidase, Legumain, AEP, AI746452, AU022324, Prsc1, ATGP3295-10 µg, ATGP3295-20 µg, ATGP3295-50 µg, ATGP3295-100 µg, ATGP3295-250 µg, ATGP3295-500 µg, ATGP3295-1 mg, ATGP3295-10, ATGP3295-20, ATGP3295-50, ATGP3295-100, ATGP3295-250, ATGP3295-500, ATGP3295-1

Shirahama-Noda K., et al. (2003) J. Biol. Chem. 278:33194-33199.; ; Liu C., et al. (2003) Cancer Res. 63:2957-2964.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_035305

O89017

Mouse

Val18

VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMM NHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTA HDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHYLE HHHHHH