Recombinant muis LYPLA1-eiwit

LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity. Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.8 kDa (250aa) confirmed by MALDI-TOF

LYPLA1, Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1, ATGP0720-10 µg, ATGP0720-20 µg, ATGP0720-50 µg, ATGP0720-100 µg, ATGP0720-250 µg, ATGP0720-500 µg, ATGP0720-1 mg, ATGP0720-10, ATGP0720-20, ATGP0720-50, ATGP0720-100, ATGP0720-250, ATGP0720-500, ATGP0720-1

Schaloske R.H., et al. (2006) Biochim Biophys Acta. 1761:1246-1259.; ; Hiraoka M., et al. (2002) J. Biol. Chem 277:10090-10099.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Metabolism

NP_032892

P97823

Mouse

MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID