Recombinant muis M-CSF-eiwit

M-CSF, also known as macrophage colony-stimulating factor 1 isoform 1, is one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhancesantibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption byosteoclasts. It plays an essential role in the regulation of survival, proliferation and differentiation ofhematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. This proteinpromotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and ininflammatory processes. Recombinant mouse M-CSF, fused to His-tag at C-terminus, was expressed in insect celland purified by using conventional chromatography techniques.

Mouse M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range is ≤4 ng/mL.

M-Csf, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csf1, C87615, MCSF, op, Processedmacrophage colony-stimulating factor 1

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is ≤4 ng/mL.

19.1 kDa (164aa)

M-CSF, ATGP3904-10 µg, ATGP3904-20 µg, ATGP3904-50 µg, ATGP3904-100 µg, ATGP3904-250 µg, ATGP3904-500 µg, ATGP3904-1 mg, ATGP3904-10, ATGP3904-20, ATGP3904-50, ATGP3904-100, ATGP3904-250, ATGP3904-500, ATGP3904-1

Richardsen E., et al. (2018) Anticancer Res. 35 (2) :865-874.; ; Braza MS., et al. (2018) Am J Transplant. 18:1247-1255.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_031804

P07141

Mouse

Ala

KEVSEHC SHMIGNGHLK VLQQLIDSQM ETSCQIAFEF VDQEQLDDPV CYLKKAFFLV QDIIDETMRF KDNTPNANAT ERLQELSNNL NSCFTKDYEE QNKACVRTFH ETPLQLLEKI KNFFNETKNL LEKDWNIFTK NCNNSFAKCS SRDVVTKP