Recombinant muis Meprin beta Subunit/MEP1B eiwit

MEP1B, also known as meprin A subunit beta, is a member of the astacin family of zinc endopeptidases 1, 2. It is disulfide-linked, tetrameric metalloendopeptidases in renal brush border membranes. It is highly regulated, secreted, and cell-surface metalloendopeptidases that are abundantly expressed in the kidney and intestine. Recombinant mouse MEP1B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Meprin A subunit beta, Mep1b, Mep-1b

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

72.6 kDa (642aa)

Meprin b Subunit, MEP1B, Meprin A subunit beta, Mep1b, Mep-1b, ATGP3323-10 µg, ATGP3323-20 µg, ATGP3323-50 µg, ATGP3323-100 µg, ATGP3323-250 µg, ATGP3323-500 µg, ATGP3323-1 mg, ATGP3323-10, ATGP3323-20, ATGP3323-50, ATGP3323-100, ATGP3323-250, ATGP3323-500, ATGP3323-1

Bertenshaw GP., et al. (2001) J Biol Chem. 276:13248-13255.; ; Kounnas MZ., et al. (1991) J Biol Chem. 266:17350-17357.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_032612

Q61847

Mouse

Leu21

LPAPEKFVKD IDGGIDQDIF DINQGLGLDL FEGDIKLEAN GKNSIIGDHK RWPHTIPYVL EDSLEMNAKG VILNAFERYR LKTCIDFKPW SGEANYISVF KGSGCWSSVG NIHAGKQELS IGTNCDRIAT VQHEFLHALG FWHEQSRADR DDYVIIVWDR IQPGKEHNFN IYNDSVSDSL NVPYDYTSVM HYSKTAFQNG TESTIVTRIS EFEDVIGQRM DFSDYDLLKL NQLYNCTSSL SFMDSCDFEL ENICGMIQSS GDSADWQRVS QVLSGPESDH SKMGQCKDSG FFMHFNTSIL NEGATAMLES RLLYPKRGFQ CLEFYLYNSG SGNDQLNIYT REYTTGQQGG VLTLQRQIKE VPIGSWQLHY VTLQVTKKFR VVFEGLRGPG TSSGGLSIDD INLSETRCPH HIWHIQNFTQ ILGGQDTSVY SPPFYSSKGY AFQIYMDLRS STNVGIYFHL ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMFNQRSIT TDPTMTSDNG SYFWDRPSKV GVTDVFPNGT QFSRGIGYGT TVFITRERLK SREFIKGDDI YILLTVEDIS HLNSTSAVPD PVPTLAVHNA CSEVVCQNGG ICVVQDGRAE CKCPAGEDWW YMGKRCEKRG STRDVEHHHH HH