Recombinant muis MIF-eiwit

Mif also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant mouse Mif, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Macrophage migration inhibitory factor, GIF, Glif

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.9 kDa (138aa) confirmed by MALDI-TOF

MIF, Macrophage migration inhibitory factor, GIF, Glif, ATGP3091-10 µg, ATGP3091-20 µg, ATGP3091-50 µg, ATGP3091-100 µg, ATGP3091-250 µg, ATGP3091-500 µg, ATGP3091-1 mg, ATGP3091-10, ATGP3091-20, ATGP3091-50, ATGP3091-100, ATGP3091-250, ATGP3091-500, ATGP3091-1

Miyatake S., et al. (2014) Biochem. Biophys. Res. Commun. 444 (4), 496-501; ; Muller I., et al. (2013) J. Biol. Chem. 288 (44), 31635-31645

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Hormones & Cytokines

NP_034928

P34884

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMPMFIVN TNVPRASVPE GFLSELTQQL AQATGKPAQY IAVHVVPDQL MTFSGTNDPC ALCSLHSIGK IGGAQNRNYS KLLCGLLSDR LHISPDRVYI NYYDMNAANV GWNGSTFA