Recombinant muis Neutrofiel Elastase/ELA2-eiwit

Neutrophil Elastase, also known as polymorphonuclear leukocyte eslastase, is a serine protease that classified as the chymotrypsin family. This protein breaks down elastin, an elastic fiber together with collagen and determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Also, this protein is inhibited by the acute-phase protein alpha 1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin. A1AT is normally secreted by the liver cells into the serum. Alpha-1-antitrypsin deficiency (A1AD) leads to uninhibited destruction of elastic fiber by elastase. Recombinant Mouse Neutrophil Elastase, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Elane, Ela2, F430011M15Rik, NE, Neutrophil elastase, Elastase-2, Leukocyte elastase

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

26.8 kDa (245aa)

Neutrophil Elastase, ELA2, ATGP3873-10 µg, ATGP3873-20 µg, ATGP3873-50 µg, ATGP3873-100 µg, ATGP3873-250 µg, ATGP3873-500 µg, ATGP3873-1 mg, ATGP3873-10, ATGP3873-20, ATGP3873-50, ATGP3873-100, ATGP3873-250, ATGP3873-500, ATGP3873-1

Wiesmeier M., et al, (2016) PLoS ONE 11:e0168055.; ; Sturrock A., et al, (1998) Cytogenet. Cell Genet. 83:104-108.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol

Immunology

NP_056594.2

Q3UP87

Mouse

Ser27