Recombinant muis Nicotinamide N-methyltransferase/NNMT-eiwit

Nnmt, also known as Nicotinamide N-methyltransferase isoform1, is the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Nnmt catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant mouse Nnmt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nicotinamide N-methyltransferase isoform1

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

32.1 kDa (288aa) confirmed by MALDI-TOF

Nnmt, Nicotinamide N-methyltransferase isoform1, ATGP3589-10 µg, ATGP3589-20 µg, ATGP3589-50 µg, ATGP3589-100 µg, ATGP3589-250 µg, ATGP3589-500 µg, ATGP3589-1 mg, ATGP3589-10, ATGP3589-20, ATGP3589-50, ATGP3589-100, ATGP3589-250, ATGP3589-500, ATGP3589-1

Parsons RB., et al. (2002) . J Neuropathol Exp Neurol. 61 (2) :111-24.; ; Smith ML., et al. (1998) . Biochim Biophys Acta. 1442 (2-3) :238-44.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_035054

O55239

Mouse

MGSSHHHHHH SSGLVPRGSH MGSHMESGFT SKDTYLSHFN PRDYLEKYYS FGSRHCAENE ILRHLLKNLF KIFCLGAVKG ELLIDIGSGP TIYQLLSACE SFTEIIVSDY TDQNLWELQK WLKKEPGAFD WSPVVTYVCD LEGNRMKGPE KEEKLRRAIK QVLKCDVTQS QPLGGVSLPP ADCLLSTLCL DAACPDLPAY RTALRNLGSL LKPGGFLVMV DALKSSYYMI GEQKFSSLPL GWETVRDAVE EAGYTIEQFE VISQNYSSTT SNNEGLFSLV GRKPGRSE