Recombinant muis OGG1-eiwit

Ogg1, also known as N-glycosylase/DNA lyase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS) . It has a beta lyase activity that nicks DNA 3 to the lesion. Recombinant mouse Ogg1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

N-glycosylase/DNA lyase, Mmh, 8-oxoguanine DNA-glycosylase 1, DNA-apurinic or apyrimidinic site lyase, AP lyase

E.coli

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

41.3 kDa (368aa) Confirmed by MALDI-TOF

Ogg1, N-glycosylase/DNA lyase, Mmh, ATGP3602-10 µg, ATGP3602-20 µg, ATGP3602-50 µg, ATGP3602-100 µg, ATGP3602-250 µg, ATGP3602-500 µg, ATGP3602-1 mg, ATGP3602-10, ATGP3602-20, ATGP3602-50, ATGP3602-100, ATGP3602-250, ATGP3602-500, ATGP3602-1

Seeberg E., et al. (2002) . Nucleic Acids Res. 30 (11) :2349-57.; ; Hodges NJ., et al (2010) . DNA Repair (Amst) . 9 (2) :144-52.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol

Enzymes & Proteases

NP_035087

O08760

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMLFRSWL PSSMRHRTLS SSPALWASIP CPRSELRLDL VLASGQSFRW KEQSPAHWSG VLADQVWTLT QTEDQLYCTV YRGDDSQVSR PTLEELETLH KYFQLDVSLA QLYSHWASVD SHFQRVAQKF QGVRLLRQDP TECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPNLHAL AGPEAETHLR KLGLGYRARY VRASAKAILE EQGGPAWLQQ LRVAPYEEAH KALCTLPGVG AKVADCICLM ALDKPQAVPV DVHVWQIAHR DYGWHPKTSQ AKGPSPLANK ELGNFFRNLW GPYAGWAQAV LFSADLRQPS LSREPPAKRK KGSKRPEG