Recombinant muis P4HB eiwit

P4HB, also known as protein disulfide-isomerase, prolyl 4-hydroxylase subunit beta, protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase, is an abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Recombinant mouse P4HB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is >120 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

P4HB, ERp59, PDI, Pdia1, Thbp, Protein disulfide-isomerase, Cellular thyroid hormone-binding protein, p55

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is >120 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

56.1 kDa (498aa)

P4HB, P4HB, ERp59, PDI, Pdia1, Thbp, ATGP3297-10 µg, ATGP3297-20 µg, ATGP3297-50 µg, ATGP3297-100 µg, ATGP3297-250 µg, ATGP3297-500 µg, ATGP3297-1 mg, ATGP3297-10, ATGP3297-20, ATGP3297-50, ATGP3297-100, ATGP3297-250, ATGP3297-500, ATGP3297-1

Wetterau J., et al. (1990) J. Biol. Chem. 265:9800-9807.; ; Sliskovic I., et al. (2005) J. Biol. Chem. 280:8733-8741.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_035162

P09103

Mouse

Asp20