Recombinant muis PD-L1/B7-H1-eiwit

CD274, also known as programmed cell death 1 ligand 1 (PDCD1L1), is involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. This protein interacts with PDCD1 inhibits T-cell proliferation and cytokine production. Recombinant mouse CD274 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

CD274 antigen, A530045L16Rik, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

27.6 kDa (246aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PD-L1, B7-H1, CD274 antigen, A530045L16Rik, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1, ATGP1645-10 µg, ATGP1645-20 µg, ATGP1645-50 µg, ATGP1645-100 µg, ATGP1645-250 µg, ATGP1645-500 µg, ATGP1645-1 mg, ATGP1645-10, ATGP1645-20, ATGP1645-50, ATGP1645-100, ATGP1645-250, ATGP1645-500, ATGP1645-1

Li, X., et al. (2012) J. Immunol. 188 (2), 724-734; ; Pulko, V., et al. (2011) J. Immunol. 187 (11), 5606-5614

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Immunology

NP_068693

Q9EP73

Mouse

FTITA PKDLYVVEYG SNVTMECRFP VERELDLLAL VVYWEKEDEQ VIQFVAGEED LKPQHSNFRG RASLPKDQLL KGNAALQITD VKLQDAGVYC CIISYGGADY KRITLKVNAP YRKINQRISV DPATSEHELI CQAEGYPEAE VIWTNSDHQP VSGKRSVTTS RTEGMLLNVT SSLRVNATAN DVFYCTFWRS QPGQNHTAEL IIPELPATHP PQNRTH