Recombinant muis Peroxiredoxin 1/PRDX1-eiwit

Prdx1, also known as peroxiredoxin-1, is an important member of peroxiredoxins (Prdxs) regulating various cellular signaling and differentiation. It confers an aggressive survival phenotype of cancer cells and drug-resistance. This protein is also identified as a red blood cell factor which enhances natural killer (NK) cell activity. Recombinant mouse Prdx1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is >2,500 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

Macrophage 23kDa stress protein, Macrophase stress protein 22kDa, MSP23, OSF-3, Osteoblast specific factor 3, PAG, Paga, prx1, PrxI, Prx I, Tdpx2, TDX2, Thioredoxin dependent peroxide reductase 2, Trx dependent peroxide reductase 2

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is >4,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

23.2 kDa (207aa)

Peroxiredoxin1, Prdx1, Peroxiredoxin-1, Prdx1, MSP23, NkefA, OSF-3, OSF3, PAG, Paga, Prdx I, prdx1, Prx I, Tdpx2, TDX2, TPxA, ATGP3340-10 µg, ATGP3340-20 µg, ATGP3340-50 µg, ATGP3340-100 µg, ATGP3340-250 µg, ATGP3340-500 µg, ATGP3340-1 mg, ATGP3340-10, ATGP3340-20, ATGP3340-50, ATGP3340-100, ATGP3340-250, ATGP3340-500, ATGP3340-1

Zhou J., et al. (2015) Int J Clin Exp Pathol. 8:9863-9874.; ; Sauri H., et al. (1996) J Leukoc Biol. 59:925-931.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_035164

P35700

Mouse

Met1