Recombinant muis Peroxiredoxin 2/PRDX2-eiwit

Prdx2, also known as peroxiredoxin-2, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Prdx2 may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8 (+) T-cells. If Prdx2 protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer's or DNA damage leading to cancer. Recombinant mouse Prdx2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 3,000 pmol/min/ug, Activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

Protector protein, PRP, PrxII, Prx II-1, Tdpx1, TDX1, Thiol specific antioxidant protein, Thioredoxin dependent peroxide reductase 1, Thioredoxin peroxidase, Thioredoxin reductase, TPx, TR, Trx dependent peroxide reductase 1, TSA

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

Specific activity is > 3,000pmol/min/ug, Activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

24.3 kDa (222aa) Confirmed by MALDI-TOF

AL022839, Band 8, Band8, Band-8, NkefB, Peroxiredoxin 2, Peroxiredoxin2, Peroxiredoxin-2, Prdx 2, Prdx2, Prdx-2, Protector protein, PRP, Prx II 1, Prx II-1, PrxII, Tdpx 1, Tdpx1, Tdpx-1, TDX 1, TDX1, TDX-1, Thiol specific antioxidant protein, Thioredoxin dependent peroxide reductase 1, Thioredoxin peroxidase, Thioredoxin reductase, Torin, TPx, TPx B, TPx-B, TR, Trx dependent peroxide reductase 1, TSA, ATGP3684-10 µg, ATGP3684-20 µg, ATGP3684-50 µg, ATGP3684-100 µg, ATGP3684-250 µg, ATGP3684-500 µg, ATGP3684-1 mg, ATGP3684-010, ATGP3684-020, ATGP3684-050, ATGP3684-100, ATGP3684-250, ATGP3684-500, ATGP3684-01M

Kim H S., et al (2009) . Oncol Rep. 21 (6) :1391-6.; ; Kim J H., et al (2008) . Clin Cancer Res. 14 (8) :2326-33.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Redox Proteins

NP_035693

Q61171

Mouse

MASGNA QIGKSAPDFT ATAVVDGAFK EIKLSDYRGK YVVLFFYPLD FTFVCPTEII AFSDHAEDFR KLGCEVLGVS VDSQFTHLAW INTPRKEGGL GPLNIPLLAD VTKSLSQNYG VLKNDEGIAY RGLFIIDAKG VLRQITVNDL PVGRSVDEAL RLVQAFQYTD EHGEVCPAGW KPGSDTIKPN VDDSKEYFSK HN