Recombinant muis PGAM1-eiwit

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.4 kDa (278aa) confirmed by MALDI-TOF

PGAM1, Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1, ATGP1496-10 µg, ATGP1496-20 µg, ATGP1496-50 µg, ATGP1496-100 µg, ATGP1496-250 µg, ATGP1496-500 µg, ATGP1496-1 mg, ATGP1496-10, ATGP1496-20, ATGP1496-50, ATGP1496-100, ATGP1496-250, ATGP1496-500, ATGP1496-1

Cao L., et al. (2007) J. Immunol. 179:5864-5876; ; Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Cancer

NP_075907

Q9DBJ1

Mouse

MAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK