Recombinant muis PGAM1-eiwit

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is >150unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

31.4 kDa (278aa) confirmed by MALDI-TOF

PGAM1, Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1, ATGP3427-10 µg, ATGP3427-20 µg, ATGP3427-50 µg, ATGP3427-100 µg, ATGP3427-250 µg, ATGP3427-500 µg, ATGP3427-1 mg, ATGP3427-10, ATGP3427-20, ATGP3427-50, ATGP3427-100, ATGP3427-250, ATGP3427-500, ATGP3427-1

Cao L., et al. (2007) J. Immunol. 179:5864-5876; ; Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Cancer

NP_075907

Q9DBJ1

Mouse

MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK