Recombinant muis PGK1-eiwit

Pgk1 also known as Phosphoglycerate kinase 1 is an X-linked enzyme that plays a key role in the glycolytic pathway. Pgk1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. This protein catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant mouse Pgk1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Phosphoglycerate kinase 1, Pgk-1

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity: > 500unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

47.1 kDa (441aa) confirmed by MALDI-TOF

Pgk1, Phosphoglycerate kinase 1, Pgk-1, ATGP3072-10 µg, ATGP3072-20 µg, ATGP3072-50 µg, ATGP3072-100 µg, ATGP3072-250 µg, ATGP3072-500 µg, ATGP3072-1 mg, ATGP3072-10, ATGP3072-20, ATGP3072-50, ATGP3072-100, ATGP3072-250, ATGP3072-500, ATGP3072-1

Chauvin T., et al. (2012) Biol. Reprod. 87 (6), 141

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Cardiovascular

NP_032854

P09411

Mouse

MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V