Recombinant muis PGP9.5/UCHL1-eiwit

Uchl1 also known as Ubiquitin carboxyl-terminal hydrolase isozyme L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. Recombinant mouse Uchl1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 70 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C

Ubiquitin carboxyl-terminal hydrolase isozyme L1, AW822034, C88048, gad, PGP 9.5, PGP9.5, R75593, UCH-L1, UCHL-1, Neuron cytoplasmic protein 9.5, Ubiquitin thioesterase L1

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 70pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37C

27.2 kDa (246aa) confirmed by MALDI-TOF

UCH-L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, AW822034, C88048, gad, PGP 9.5, PGP9.5, R75593, UCH-L1, UCHL-1, ATGP3568-10 µg, ATGP3568-20 µg, ATGP3568-50 µg, ATGP3568-100 µg, ATGP3568-250 µg, ATGP3568-500 µg, ATGP3568-1 mg, ATGP3568-10, ATGP3568-20, ATGP3568-50, ATGP3568-100, ATGP3568-250, ATGP3568-500, ATGP3568-1

Wiese CB., et al. (2013) Genesis 51 (12), 852-861

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Neuroscience

NP_035800

Q9R0P9

Mouse

MQLKPME INPEMLNKVL AKLGVAGQWR FADVLGLEEE TLGSVPSPAC ALLLLFPLTA QHENFRKKQI EELKGQEVSP KVYFMKQTIG NSCGTIGLIH AVANNQDKLE FEDGSVLKQF LSETEKLSPE DRAKCFEKNE AIQAAHDSVA QEGQCRVDDK VNFHFILFNN VDGHLYELDG RMPFPVNHGA SSEDSLLQDA AKVCREFTER EQGEVRFSAV ALCKAA