Recombinant muis Pin1-eiwit

Pin1, also known as peptidyl-prolyl cis-trans isomerase NIMA-interacting1, is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. Recombinant mouse PIN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 1,200 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Peptidyl-prolyl cis-trans isomerase NIMA-interacting1, 0610025L01Rik, D9Bwg1161e

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 1,200 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

20.8 kDa (188aa) Confirmed by MALDI-TOF

Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting1, 0610025L01Rik, D9Bwg1161e, ATGP3566-10 µg, ATGP3566-20 µg, ATGP3566-50 µg, ATGP3566-100 µg, ATGP3566-250 µg, ATGP3566-500 µg, ATGP3566-1 mg, ATGP3566-10, ATGP3566-20, ATGP3566-50, ATGP3566-100, ATGP3566-250, ATGP3566-500, ATGP3566-1

Wulf GM., et al. (2002) J Biol Chem. 277 (50) :47976-9; ; Zacchi P., et al. (2002) Nature. 419 (6909) : 853-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_075860

Q9QUR7

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMADEEKL PPGWEKRMSR SSGRVYYFNH ITNASQWERP SGGSTVGGSS KNGQGEPAKV RCSHLLVKHS QSRRPSSWRQ EKITRSKEEA LELINGYIQK IKSGEEDFES LASQFSDCSS AKARGDLGPF SRGQMQKPFE DASFALRTGE MSGPVFTDSG IHIILRTE