Recombinant muis PKM eiwit

Pkm, also known as pyruvate kinase PKM isoform M2, is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. Pkm interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant mouse Pkm, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C

Pyruvate kinase PKM isoform M2, Pyruvate kinase muscle isozyme, pyruvate kinase M1/2, Threonine-protein kinase PKM2, Tyrosine-protein kinase PKM2, Pk3, Pkm2, Pykm

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

> 85% by SDS-PAGE

Specific activity: > 50,000pmol/min/ug. One unit will convert 1.0pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C

60.2 kDa (554aa) Confirmed by MALDI-TOF

Pkm, Pyruvate kinase PKM isoform M2, AA414905, AL024370, AL024424, Pk-2, Pk-3, Pk3, Pkm2, ATGP3610-10 µg, ATGP3610-20 µg, ATGP3610-50 µg, ATGP3610-100 µg, ATGP3610-250 µg, ATGP3610-500 µg, ATGP3610-1 mg, ATGP3610-10, ATGP3610-20, ATGP3610-50, ATGP3610-100, ATGP3610-250, ATGP3610-500, ATGP3610-1

Spoden GA., et al. (2009) . Exp Cell Res. 315 (16) :2765-74; ; Mazurek S., et al. (2007) . Ernst Schering Found Symp Proc. (4) :99-124.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol

Cancer

NP_035229

P52480

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP