Recombinant muis Podocalyxin-eiwit

PODXL, also known as Podocalyxin, is a heavily glycosylated transmembrane sialoprotein in the CD34 and endoglycan family. This protein involved in the regulation of both adhesion and cell morphology and cancer progression. It has to function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Also, it acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Recombinant mouse PODXL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Podocalyxin, AW121214, Ly102, PC, PCLP-1, Pclp1

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

41kDa (391aa)

Podocalyxin, Podocalyxin, AW121214, Ly102, PC, PCLP-1, Pclp1, ATGP3311-10 µg, ATGP3311-20 µg, ATGP3311-50 µg, ATGP3311-100 µg, ATGP3311-250 µg, ATGP3311-500 µg, ATGP3311-1 mg, ATGP3311-10, ATGP3311-20, ATGP3311-50, ATGP3311-100, ATGP3311-250, ATGP3311-500, ATGP3311-1

Vitureira N., et al. (2010) PLoS ONE 8:e12003.; ; Nielsen JS., et al. (2007) PLoS ONE 2:E237.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_038751

Q9R0M4

Mouse

His22

HNGNETSTSA IKSSTVQSHQ SATTSTEVTT GHPVASTLAS TQPSNPTPFT TSTQSPSMPT STPNPTSNQS GGNLTSSVSE VDKTKTSSPS STAFTSSSGQ TASSGGKSGD SFTTAPTTTL GLINVSSQPT DLNTTSKLLS TPTTDNTTSP QQPVDSSPST ASHPVGQHTP AAVPSSSGST PSTDNSTLTW KPTTHKPLGT SEATQPLTSQ TPGITTLPVS TLQQSMASTV GTTTEEFTHL ISNGTPVAPP GPSTPSPIWA FGNYQLNCEP PIRPDEELLI LNLTRASLCE RSPLDEKEKL VELLCHSVKA SFKPAEDLCT LHVAPILDNQ AVAVKRIIIE TKLSPKAVYE LLKDRWDDLT EAGVSDMKLG KEGPPEVNED RFSLEHHHHH H