Recombinant muis Prostaatzuur Fosfatase/ACS-eiwit

ACPP, also known as Prostatic Acid Phosphatase, is a member of histidine acid phosphatase family. Prostatic Acid Phosphatase/ACPP catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins. The activity optimum of Prostatic Acid Phosphatase/ACPP is in the pH range of 4 - 6, and the activity is inhibited by L (+) -tartrate. It has also lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Recombinant mouse Prostatic Acid Phosphatase/ACPP protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Acid phosphatase, prostate, ACP3, ACP-3, ACPP, EC 3.1.3.2, PAP, Prostatic Acid Phosphatase, prostatic acid phosphatase, 5-nucleotidase, 5'-NT, Acid phosphatase 3, Ecto-5'-nucleotidase, Fluoride-resistant acid phosphatase, FRAP, Thiamine monophosphatase, TMPase, A030005E02Rik, Lap, PAP, Ppal

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

41.3kDa (356aa)

, ATGP3953-10 µg, ATGP3953-20 µg, ATGP3953-50 µg, ATGP3953-100 µg, ATGP3953-250 µg, ATGP3953-500 µg, ATGP3953-1 mg, ATGP3953-10, ATGP3953-20, ATGP3953-50, ATGP3953-100, ATGP3953-250, ATGP3953-500, ATGP3953-1

Lin, M.F. and G.M. Clinton (1986) Biochem. J. 235:351-357.; ; Veeramani, S. et al. (2005) Endocr. Relat. Cancer 12:805-822.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases, Cell Cycle

NP_062781

Q8CE08

Mouse

Lys32