Recombinant muis RalA-eiwit

Rala, also known as Ras-related protein Ral-A, is a member of the small GTPase superfamily, Ras family of proteins. Rala mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. Rala is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis. Recombinant mouse Rala protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein Ral-A, 3010001O15Rik, AW322615, Ral, Rasl1, V-ral simian leukemia viral oncogene A, RAS like proto-oncogene A

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

25.7 kDa (227aa) confirmed by MALDI-TOF

V-ral simian leukemia viral oncogene A, V ral simian leukemia viral oncogene A, Ras-related protein Ral-A, Rasl1, Rasl 1, Ras related protein Ral A, RAS like proto-oncogene A, RAS like proto oncogene A, Rala, Ral, AW322615, 3010001O15Rik, , ATGP3699-10 µg, ATGP3699-20 µg, ATGP3699-50 µg, ATGP3699-100 µg, ATGP3699-250 µg, ATGP3699-500 µg, ATGP3699-1 mg, ATGP3699-010, ATGP3699-020, ATGP3699-050, ATGP3699-100, ATGP3699-250, ATGP3699-500, ATGP3699-01M

Cascone I., et al. (2008) EMBO J. 27 (18) :2375-2387.; ; Balasubramanian N., et al. (2010) Curr. Biol. 20 (1) :75-79.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 30% glycerol, 0.1M NaCl, 0.1mM PMSF

Signal Transduction

NP_062364

P63321

Mouse

MGSSHHHHHH SSGLVPRGSH MGSHMAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA DQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC