Recombinant muis Recoverin eiwit

Recoverin also known as Rcvrn, is a heterogeneously acylated calcium-binding and intracellular signal transduction protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recently, recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome. Recombinant Mouse recoverin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Recoverin, CAR, S-modulin

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

25.8 kDa (225aa)

Recoverin, Recoverin, CAR, S-modulin, ATGP3808-10 µg, ATGP3808-20 µg, ATGP3808-50 µg, ATGP3808-100 µg, ATGP3808-250 µg, ATGP3808-500 µg, ATGP3808-1 mg, ATGP3808-10, ATGP3808-20, ATGP3808-50, ATGP3808-100, ATGP3808-250, ATGP3808-500, ATGP3808-1

Sampath P., et al. (2004) Cancer Control. 11 (3) : 174-80; ; Erickson MA., et al. (1998) Proc. Natl. Acad. Sci. 95 (11) : 6474-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Cancer

NP_033064

P34057

Mouse

KSG ALSKEILEEL QLNTKFTEEE LSAWYQSFLK ECPSGRITRQ EFESIYSKFF PDSDPKAYAQ HVFRSFDANS DGTLDFKEYV IALHMTTAGK PTQKLEWAFS LYDVDGNGTI SKNEVLEIVM AIFKMIKPED VKLLPDDENT PEKRAEKIWA FFGKKEDDKL TEEEFIEGTL ANKEILRLIQ FEPQKVKERI KEKKQ