Recombinant muis Renine-eiwit

REN1, also known as Renin-1, is a member of the peptidase A1 family. It is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. Androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. This protein is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Also, REN1 was found to be co localized with the lysosomal marker, beta-glucuronidase, by double-fluorescent labeling. Recombinant mouse REN1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Ren1, Ren, Ren-A, Ren1c, Ren1d, Rn-1, Rnr, Angiotensinogenase, Kidney renin

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

42.5 kDa (387aa)

Renin, Ren1, Ren, Ren-A, Ren1c, Ren1d, Rn-1, Rnr, Angiotensinogenase, Kidney renin, ATGP3224-10 µg, ATGP3224-20 µg, ATGP3224-50 µg, ATGP3224-100 µg, ATGP3224-250 µg, ATGP3224-500 µg, ATGP3224-1 mg, ATGP3224-10, ATGP3224-20, ATGP3224-50, ATGP3224-100, ATGP3224-250, ATGP3224-500, ATGP3224-1

Ramkumar N., et al. (2013) Am. J. Hypertens. 26 (8) :965-972.; ; Bandulik S., et al. (2013) Endocrinology 154 (8) :2712-2722.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_112469

P06281

Mouse