Recombinant muis RKIP/PEBP1-eiwit

Pebp1 also known as Phosphatidylethanolamine-binding protein 1. The protein Binds with ATP, opioids and phosphatidylethanolamine. Pebp1 exerts inhibitory activity against several serine proteases including thrombin, neuropsin, and chymotrypsin, it also inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Also, targeting Pebp1 levels can delay photoreceptor degeneration, assisting in extending the time-window for treating such rapidly progressing blindness disorder. Recombinant mouse Pebp1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphatidylethanolamine-binding protein 1, HCNP, Pbp, Pbp1, Pbqr, Rkip, prostatic binding protein, Raf kinase inhibitory protein, hippocampal cholinergic neurostimulating peptide

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

23.2 kDa (210aa) confirmed by MALDI-TOF

Rkip, Raf kinase inhibitory protein, Prostatic binding protein, Phosphatidylethanolamine-binding protein 1, Phosphatidylethanolamine binding protein 1, Pbqr, Pbp1, Pbp 1, Pbp, Hippocampal cholinergic neurostimulating peptide, HCNP, ATGP3172-10 µg, ATGP3172-20 µg, ATGP3172-50 µg, ATGP3172-100 µg, ATGP3172-250 µg, ATGP3172-500 µg, ATGP3172-1 mg, ATGP3172-010, ATGP3172-020, ATGP3172-050, ATGP3172-100, ATGP3172-250, ATGP3172-500, ATGP3172-01M

Hengst U., et al. (2001) J Biol Chem. 276 (1) :535-40; ; Subramanian B., et al. (2014) Invest Ophthalmol Vis Sci. 55 (9) :5788-94

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_061346

P70296

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMAADISQ WAGPLCLQEV DEPPQHALRV DYAGVTVDEL GKVLTPTQVM NRPSSISWDG LDPGKLYTLV LTDPDAPSRK DPKFREWHHF LVVNMKGNDI SSGTVLSDYV GSGPPSGTGL HRYVWLVYEQ EQPLSCDEPI LSNKSGDNRG KFKVETFRKK YNLGAPVAGT CYQAEWDDYV PKLYEQLSGK